① Background
The incidence and mortality of infectious diseases are both among the top ten in the world, and accurate detection of pathogens in clinical samples is the only way to develop the most accurate treatment strategy. Due to the large variety of pathogenic microorganisms and the low throughput and time-consuming nature of traditional clinical methods, about 50% of infected patients are still facing difficulties in pathogen diagnosis. This project achieves extensive and unbiased detection of pathogenic microorganisms by sequencing all the nucleic acids in the samples.
② Technology
Adopt the fourth-generation high-throughput sequencing technology of ONT platform - Nanopore single-molecule sequencing technology, combined with the advantages of targeted amplification of bacteria, fungi, mycobacteria, special pathogens (atypical pathogens), viruses and other pathogenic microorganisms in specific gene regions and the technology itself has long reads, real-time data output, etc., it can accurately identify to the species or more detailed subspecies, which helps to accurately and efficiently detect a variety of microorganisms in clinical samples, and the report provides all microorganisms that can be detected in the sample with valid data, and assists clinicians in analyzing and judging the current infection situation through report interpretation.
③ Content
Test project name | Detection range |
MTseq broad-spectrum pathogen detection | Supports detection of over 17,000 bacterial species (including 268 special pathogens), more than 8,900 fungal species, and 60 viral species, enabling the detection of a total of over 20,000 pathogenic microorganisms. |
TBseek-Mycobacterium tuberculosis and its drug resistance gene detection | Support for detecting Mycobacterium tuberculosis complex, 40 species of non-tuberculous mycobacteria, 6 resistance genes related to 4 first-line tuberculosis drugs, 13 resistance genes related to 11 second-line tuberculosis drugs. |
HIV SEEK-HIV typing and drug resistance gene testing | Support for a single test to detect HIV-1 and HIV-2 and to distinguish multiple subtypes of HIV-1 and related drug-resistant genes |
④ Advantages
a. High sensitivity: By targeting the amplification of the pathogen nucleic acid, it can detect special samples with low abundance pathogens in a high human background, such as blood infection samples; it can detect infected samples with low sample volume, such as ophthalmic infections.
b. Achieve comprehensive pathogen diagnosis on the same day, with a preliminary report within 2 hours and a final report time of less than 16 hours.
c. Low false positive rate: Long-read sequencing, no assembly required, not affected by host nucleic acid, achieving precise localization of infectious pathogens.
d. Comprehensive detection range: Supports the simultaneous detection of DNA and RNA, enabling the identification of approximately 20,000 types of pathogenic microorganisms, including viruses, fungi, bacteria, and special pathogens, thus achieving comprehensive and unbiased pathogenic microbial detection.
⑤ Detection process
⑥ Applicable people
Patients with clinical fever or infection of unknown cause
Patients with negative clinical auxiliary etiology examination results
Patients suspected or confirmed of infection with rare pathogens
Patients suspected or confirmed of mixed infection
Patients with known definite infectious agents, for disease course monitoring